Mass spectrometry equipment
Waters SynaptTM HDMS
Type: Quadrupole (TWIMS) Time-of-flight
Date acquired: 2008
Comments: Triwave technology with high efficiency ion mobility spectrometry enabling sample separation based on molecule's mass as well as their size, shape and charge
Sources: Electrospray (ESI), nanospray and lockspray
Use: Ion mobility study, time aligned parallel fragmentation for structural analysis, high mass protein and DNA research and accurate mass analysis
Waters Qtof Ultima
Type: Quadrupole Time-of-flight
Date acquired: 2004
Comments: Factory modified for high mass/charge transmission
Sources: Electrospray (ESI), nanoESI, lockspray
Use: High mass protein analysis, binding interactions and DNA research. Accurate mass analysis
Waters Quattro Micro
Type: Triple Quadrupole
Date acquired: 2004 (as 2nd hand instrument)
Sources: Electrospray (ESI), nanESI
Use: Gas phase thermochemistry, reaction monitoring and lipid research
Waters Xevo QTOF
Type: Quadrupole time-of-flight
Date acquired: 2010
Sources: Electrospray (ESI), Lockspray and Atmospheric Solids Analysis Probe (ASAP)
Use: Accurate mass analysis, high resolution mass spectrometry, tandem mass spectrometry
Additional Features: UPLC-MS and GC-MS
AB SCIEX QTrap 5500 With Selexion
Type: Triple quadrupole and linear ion trap
Date acquired: 2013
Comments: Fitted with selexION differential ion mobility spectrometry (DMS) device to allow separation of isobaric and co-eluting molecules. This instrument is part of a collaboration with AB Sciex (Toronto, Canada) through the ARC Linkage Program and has been modified to measure Ozone Induced Dissociation (OzID) mass spectra. OzID is a technique developed in our lab and uses a selective reaction between the ion and ozone gas to cleave double bonds and reveal their position(s) within the molecule. OzID is used routinely for structural characterisation of lipids
Sources: Electrospray
Uses: DMS separation of isomeric lipids, OzID for identification of fatty acid double bond position
Additional features: Eksigent ekspert? nanoLC400 system
AB SCIEX QTrap 5500
Type: Triple quadrupole and linear ion trap
Date acquired: 2011
Sources: Electrospray, Advion Nanomate (chip-based nanoESI)
Uses: The instrument is part of the UOW lipidomics facility and is used for the identification and quantification of a wide range of lipids in extracts from diverse sources including tissue, tears and cell culture. The automated nanomate source allows the investigation of large sample arrays
Additional features: Liquid extraction surface analysis (LESA) allowing analysis of molecules directly from a surface
Shimadzu LCMS-2010EV
Type: Single quadrupole
Date acquired: 2013
Sources: Electrospray (ESI)
Shimadzu QP5050A
Type: Gas chromatography / single quadrupole
Date acquired: 2003
Sources: Direct insertion electron impact (EI)
Use: Purity testing, low resolution compound identification
Thermo Fisher Scientific LTQ XL
Type: Linear ion trap, with coupled Surveyor LC
Date acquired: 2013
Sources: Ion Max Electrospray, 2D desorption electrospray (DESI), will be moved to this instrument soon if that counts as a source
Use: Analysis and identification of lipids and fatty acids
Thermo Fisher Scientific LTQ Orbitrap XL
Type: Linear ion trap, with orbitrap mass analyser
Date acquired: 2012
Sources: Matrix assisted laser desorption ionisation (MALDI)
Use: Accurate mass analysis of lipids and peptides. Spatial imaging of lipids in tissues