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Basic Science

Basic Science

Grant Success

  1. Peroxidation of lipids (in vitro)
    Omega-3 and omega-6 fats: Their importance and role in the biology of animals. ARC Discovery 2010-2012 (Else, Hulbert) $667k pending.

  2. Lipids (development & endurance)
    Lipidomics associated with metabolic syndrome, ageing and metabolic variation between species. ARC Linkage 2004-07 (Else, Hulbert, Blanksby) $150k.

  3. Lipid interaction, insulin signalling
    Determinants of metabolic rate in animals. ARC Discovery 2004-06 (Hulbert, Else) $180k.

  4. Lipid-Protein Interactions (molecular activity)

  5. Ether lipids (cataracts, K.O. mice, cell lines)

  6. Lipid analysis (methods)
    Unique, state-of-the-art lipidomics infrastructure. ARC LIEF 2009 (Mitchell, Blanksby, Beck, Huang, McLennan, Jessup, Gaus, Willcox, Truscott, Storlien, Caterson) 950K.

    New methods to complete the lipidomics puzzle: revealing the structural diversity of lipids by mass spectrometry. ARC Discovery 2009-11 (Blanksby, Mitchell, Ekroos) 205K

Publications

  1. Peroxidation of lipids (in vitro)

    Couldwell AM, Thomas MC, Mitchell TW, Hulbert AJ and Blanksby SJ. Tandem mass spectrometry of deprotonated iodothyronines. Rapid Communications in Mass Spectrometry 2005; 19:2295-2304.

  2. Lipids (development & endurance)

    Turner N, Lee JS, Bruce CR, Mitchell TW, Else PL, Hulbert AJ and Hawley JA. Greater effect of diet than exercise training on the fatty acid profile of rat skeletal muscle. Journal of Applied Physiology 2004; 96:974-980.

    Mitchell TW, Turner N, Hulbert AJ, Else PL, Hawley JA, Lee JS, Bruce CR and Blanksby SJ. Exercise alters the profile of phospholipid molecular species in rat skeletal muscle. Journal of Applied Physiology 2004; 97:1823-1829.

    Turner N, Hulbert AJ and Else PL (2006) Limits to physical performance and metabolism across species. Current Opinion in Clinical Nutrition and Metabolic Care 9:691-696.

  3. Lipid interaction, insulin signalling

    Cazzoli R, Mitchell TW, Burchfield J, Pedersen DJ, Turner N, Biden TJ and Schmitz-Peiffer C. Dilinoleoyl-Phosphatidic Acid Mediates Reduced IRS-1 Tyrosine Phosphorylation In Skeletal Muscle. Diabetologia 2007; 50:1732-1742.

  4. Lipid-Protein Interactions (molecular activity)

    Mitchell TW, Ekroos K, Blanksby SJ, Hulbert AJ and Else PL. Differences in membrane acyl phospholipid composition between an endothermic mammal and an ectothermic reptile are not limited to any phospholipid class. Journal of Experimental Biology 2007; 210:3440-3450.

    Nealon JR, Blanksby SJ, Mitchell TW and Else PL. Systematic changes in membrane acyl composition associated with varying body mass in mammals occur in all phospholipid classes: An analysis of kidney and brain. Journal of Experimental Biology 2008: 211:3195-3204.

    Le Lay S, Li Q, Proschogo N, Rodriguez M, Cartland S, Jessup W, Mitchell T and Gaus K. Caveolin-1-dependent and –independent membrane domains. Journal of Lipid Research 2009; 50:1609-1620.

    Turner N, Else PL and Hulbert AJ (2005) An allometric comparison of microsomal membrane lipid composition and sodium pump molecular activity in the brain of mammals and birds. Journal of Experimental Biology 208: 371-381.

    Turner N, KL Haga, AJ Hulbert & Else PL (2005) Relationship between body size, Na+-K+-ATPase activity, and membrane lipid composition in mammal and bird kidney. American Journal of Physiology 288:R301-R310.

    Starke-Peterkovic, T, N Turner, PL Else & RJ Clarke (2005) Electrical field strength of membrane lipids from vertebrate species: membrane lipid composition and Na+,-K+-ATPase molecular activity. American Journal of Physiology 288: R663-R670.

    Turner, N, AJ Hulbert and PL Else (2005) Sodium pump molecular activity and membrane lipid composition in two disparate ectotherms, and comparison with endotherms. Journal of Comparative Physiology B175:77-85.

  5. Ether lipids (cataracts, K.O. mice, cell lines)

    Deeley JM, Thomas MC, Truscott RJW, Mitchell TW and Blanksby SJ. Identification of abundant alkyl ether glycerophospholipids in the human lens by novel tandem mass spectrometry techniques. Analytical Chemistry 2009; 81:1920-1930.

    Deeley JM, Mitchell TW, Wei X, Korth J, Nealon JR, Blanksby SJ, and Truscott RJW. Human lens lipids differ markedly from those of commonly used experimental animals. Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids 2008; 1781:288-298.

    Nealon JR, Blanksby SJ, Abbott SK, Hulbert AJ, Mitchell TW and Truscott RJW. Phospholipid composition of the rat lens is independent of diet. Experimental Eye Research 2008; 87:502-514.

  6. Lipid analysis (methods)

    Mitchell TW, Pham H, Thomas MC and Blanksby SJ. Identification of Double bond position in lipids: From GC to OzID.Journal of Chromatography B 2009; 877:2722-2735.

    Thomas MC, Mitchell TW, Harman, DG, Deeley JM, Nealon JR and Blanksby SJ. Ozone Induced Dissociation (OzID): a novel method for the elucidation of double bond position within mass-selected lipid ions. Analytical Chemistry 2008; 80:303-311.

    Thomas MC, Mitchell TW, Harman DG, Deeley JM, Murphy RC and Blanksby SJ. Elucidation of double bond position in unsaturated lipids by ozone electrospray ionization mass spectrometry. Analytical Chemistry 2007; 79:5013.

    Thomas MC, Mitchell TW and Blanksby SJ. Ozonolysis of phospholipid double bonds during electrospray ionization: a new tool for structure determination. Journal of the American Chemical Society 2006; 128: 58-59.

    Thomas MC, Mitchell TW and Blanksby SJ. A comparison of the gas phase acidities of phospholipid headgroups: Experimental and computational studies. Journal of the American Society for Mass Spectrometry 2005; 16(6): 926-939.

    Mitchell TW. Tracking the phospholipid distribution of DHA by shogun lipidomics. In Lipidomics: Methods and Protocols, Armstrong D, Ed. Springer: 2009. In Press.

    Thomas MC, Mitchell TW and Blanksby SJ. On-line ozonolysis methods for the determination of double bond positions in unsaturated lipids. In Lipidomics: Methods and Protocols, Armstrong D, Ed. Springer: 2009. In Press.

    Meyer BJ. The 3 Omegas – not as easy as 1, 2, 3. Food Australia 2008;60:41-43.

    Sullivan BL, Brown J, Williams PG, Meyer BJ. Dietary validation of a new food frequency questionnaire that estimates long-chain omega-3 polyunsaturated fatty acids. British Journal of Nutrition 2008;99:660-666.

    Sullivan BL, Williams PG, Meyer BJ. Biomarker validation of a new food frequency questionnaire that estimates long-chain omega-3 polyunsaturated fatty acids Lipids, 2006;41:845-850